Effects of oxymatrine on the SIRT1/Nrf2 signaling pathway in ox-LDL-treated HUVECs. HUVECs were pretreated with oxymatrine (4 µM) for 2 h prior to treatment with ox-LDL (100 µg/ml) for 24 h. (A) Western blot analysis for SIRT1, C-Nrf2 and HO-1 protein expression. Quantitative analysis for (B) SIRT1, (C) C-Nrf2 and (D) HO-1 relative to GAPDH. (E) Western blot analysis for N-Nrf2 protein expression. (F) Quantitative analysis for Nrf2 relative to histone H3. Data are expressed as the mean ± SD, n=3. **P<0.01 and NSP>0.05 vs. the control group; #P<0.05 and ##P<0.01 vs. the ox-LDL group. OMT, oxymatrine; ox-LDL, oxidized low-density lipoprotein; NS, no statistical significance; HUVECs, human umbilical vein endothelial cells; SIRT, sirtuin; Nrf2, nuclear factor-erythroid 2-related factor 2; C-Nrf2, cytoplasmic Nrf2; N-Nrf2, nuclear Nrf2; HO-1, heme oxygenase 1.