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. 2021 Aug 4;48(4):187. doi: 10.3892/ijmm.2021.5020

Figure 4.

Figure 4

Effects of siSIRT1 transfection on the protective effects of oxymatrine against ox-LDL-induced injury in HUVECs. HUVECs transfected with siSIRT1 or siCon were treated with oxymatrine (4 µM) for 2 h prior to treatment with ox-LDL (100 µg/ml) for 24 h. (A) Western blot analysis for protein expression. Quantitative analysis for (B) SIRT1 and (C) HO-1 relative to GAPDH. (D) Quantitative analysis for N-Nrf2 relative to histone H3. Determination of (E) cell viability, (F) LDH release and (G) apoptosis rate. (H) Western blot analysis for cleaved caspase-3 and caspase-3 protein expression. (I) Quantitative analysis for cleaved caspase-3 relative to caspase-3. Data are expressed as the mean ± SD, n=3. **P<0.01 vs. the ox-LDL group; NSP>0.05 vs. the OMT + ox-LDL group; #P<0.05 and ##P<0.01 vs. the OMT + ox-LDL + siCon group. OMT, oxymatrine; ox-LDL, oxidized low-density lipoprotein; NS, no statistical significance; siSIRT1, siRNAs for SIRT1; siCon, siRNAs for negative control; HUVECs, human umbilical vein endothelial cells; LDH, lactate dehydrogenase; SIRT, sirtuin; Nrf2, nuclear factor-erythroid 2-related factor 2; C-Nrf2, cytoplasmic Nrf2; N-Nrf2, nuclear Nrf2; HO-1, heme oxygenase 1.