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. 2021 Aug 4;48(4):187. doi: 10.3892/ijmm.2021.5020

Figure 6.

Figure 6

Effects of siSIRT transfection on the inhibitory effect of oxymatrine against NLRP3 inflammasome-mediated pyroptosis in ox-LDL-treated HUVECs. HUVECs transfected with siSIRT1 or siCon were treated with oxymatrine (4 µM) for 2 h prior to treatment with ox-LDL (100 µg/ml) for 24 h. (A) Western blot analysis for protein expression. Quantitative analysis for (B) NLRP3 and (C) ASC relative to GAPDH. (D) Western blot analysis for protein expression. Quantitative analysis for (E) cleaved caspase-1/caspase-1, (F) IL-1β and (G) IL-18 relative to GAPDH. Data are expressed as the mean ± SD, n=3. **P<0.01 vs. the ox-LDL group; NSP>0.05 vs. the OMT + ox-LDL group; #P<0.05 and ##P<0.01 vs. the OMT + ox-LDL + siCon group. OMT, oxymatrine; ox-LDL, oxidized low-density lipoprotein; NS, no statistical significance; siSIRT1, siRNAs for SIRT1; siCon, siRNAs for negative control; HUVECs, human umbilical vein endothelial cells; NLRP3, NLR family pyrin domain containing 3; SIRT, sirtuin; ASC, apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain.