Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 3;48(4):185. doi: 10.3892/ijmm.2021.5018

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

HO-1^+/− knockdown mice exhibit elevated expression levels of VCAM-1 without changes in renal structure and function. (A) Genotype identification of HO^+/+ and HO^+/− mice by agarose gel electrophoresis. (B) Western blot analysis of the expression levels of HO-1, VCAM-1 and β-actin proteins in the HO-1^+/− vs. those in the wild-type mice. (C and D) Densitometric-based quantification of the western blot analysis results shown in panel B for (C) HO-1 and (D) VCAM-1 proteins using ImageJ software. Densitometry values are expressed as the mean ± SD (n=3). ^*P<0.05 vs. HO-1^+/+. (E) Immunohistochemical staining of VCAM-1-expressing cells in the kidneys of the HO-1^+/+ and HO-1^+/− mice. (F) Representative images of H&E-stained sections of renal tissue in the HO-1^+/+ and HO-1^+/− mice. (G) Tissue injury was assessed by using the scoring scale from 0 to 5 points (n=3). (H) Serum creatinine concentration in the HO-1^+/+ and HO-1^+/− mice (n=3). (I) Serum BUN concentration in the HO-1^+/+ and HO-1^+/− mice (n=3). HO-1, heme oxygenase-1; VCAM-1, vascular cell adhesion molecule-1; BUN, blood urea nitrogen.