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. 2021 Aug 12;11:581671. doi: 10.3389/fonc.2021.581671

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Copyright © 2021 Pradhan, Kumar, Surya H, Krishna, John, Jissa, Anjana, Chandramohan and Nair

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of STIL in CRC tumorigenesis. (A) Bar graph showing fold change in STIL mRNA expression upon STIL silencing. (B) Immunoblot validating STIL silencing at protein level. (C) Bar graph showing cell proliferation of HT-29 cells upon STIL silencing. (D) Histogram showing effect of STIL silencing on cell cycle. (E, F) Representative images of tumor xenograft and graph showing growth of tumor upon time (n = 3). (G) Bar graph showing mean dry weight of tumor in both groups (n = 3). (H) IHC showing nuclear expression and quantification of Ki-67 protein in xenograft derived tumor tissues. Unpaired t-test results showing p value ≤ 0.05, ≤ 0.01, ≤ 0.001 are represented by *, **, ***, respectively, Scale bar is 20 µm.