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. 2021 Aug 17;9:681968. doi: 10.3389/fcell.2021.681968

FIGURE 2.

FIGURE 2

The expression of miRNA-7062-5P upregulates during osteoclastogenesis induced by CT-26 CM and promotes osteoclast formation. (A) Scatter plots for microarray assay demonstrated differentially expressed miRNAs in early OCPs treated with CT-26 CM or DMEM. (B) The change folders of miRNA-7062-5p level in early OCPs after treatment by CT-26 CM or MC-38 CM compared with the control group. (C) The change of miRNA-7062-5p expression in sorted early OCPs at specific timepoints after injection of CT-26 cells or MC-38 cells into tibias. (D) Representative images of TRAP staining demonstrating osteoclastogenesis after early OCPs stimulated by RANKL and M-CSF in the presence of CT-26 CM after transfection with controls, agomir-7062-5p, or antagomir-7062-5p (scale bar = 250 μm). (E) Quantification of the number of TRAP positive osteoclasts per well in (D). (F) Quantification of relative TRAP activity per well in (D). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.