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. 2021 Aug 17;9:681968. doi: 10.3389/fcell.2021.681968

FIGURE 4.

FIGURE 4

Overexpression of GPR65 reverses the effect of miRNA-7062-5p in early OCPs. (A) Western blot analysis demonstrated the protein level of GPR65 in early OCPs after transfection with agomir-7062-5p alone or combination with plasmids containing GPR65 in the presence of CT-26 CM or in normal culture. (B) Quantification of relative intensity in (A). (C) Representative images of TRAP staining demonstrating osteoclast formation of early OCPs induced by RANKL and M-CSF after transfection with agomir-7062-5p alone or combination with plasmids containing GPR65 in the presence of CT-26 CM (scale bar = 250 μm). (D) Quantification of the number of osteoclasts per well in (C). (E) Quantification of relative TRAP activity per well in (C). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.