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. 2021 Aug 17;9:681968. doi: 10.3389/fcell.2021.681968

FIGURE 7.

FIGURE 7

Inhibition of miRNA-7062-5p attenuates bone resorption in bone metastatic model of CT-26 cell line in vivo. (A) Western blot analysis showed protein level of activated AMPK and JNK compared with its total protein in early OCPs sorted from antagomir-7062-5p–treated bone marrows after injection of CT-26 cells. (B) Quantification of relative intensity of phosphorylation of AMPKα compared with total AMPKαin (A). (C) Quantification of relative intensity of phosphorylation of JNK compared with total JNK in (A). (D) Upper: representative images of safranin O staining in vivo after injection with antagomir-7062-5p in bone metastatic model. Down: representative images of TRAP staining in vivo after injection with antagomir-7062-5p in bone metastatic model (scale bar = 100 μm). (E) Quantification of percentage of trabecular area in upper images in (D). (F) Quantification of percentage of TRAP-positive osteoclast surface in total trabecular surface in down images in (D). (G) Micro–computed tomography examined the bone destruction after injection with antagomir-7062-5p in bone metastatic model. (H) Quantification of bone mineral density in tibias after injection with antagomir-7062-5p in bone metastatic model. (I) Quantification of BV/TV in tibias after injection with antagomir-7062-5p in bone metastatic model. ∗p < 0.05, ∗∗p < 0.01.