Table 2.
Physical characteristics (concentration and size) of supernatants. (1) Within a certain period of culture time (30 min-24 h), extension of the culture time promoted release. (2) Coculture promoted release. (3) There were no significant differences in EV size among the supernatants.
| Sample | Size(nm) | Concentration |
|---|---|---|
| 4T1 (30 min) | 61.14 ± 16.45 | 5.45 × 109 |
| 4T1 (24 h) | 57.63 ± 16.91 | 9.47 × 109 |
| PLT (30 min) | 64.26 ± 27.31 | 2.39 × 1010 |
| PLT (24 h) | 57.35 ± 19.73 | 3.28 × 1010 |
| 4T1+PLT (30 min) | 61.79 ± 24.34 | 2.97 × 1010 |
| 4T1 + PLT (24 h) | 57.37 ± 19.55 | 5.73 × 1010 |
4T1: murine breast cancer cell line. PLT: platelets obtained from a BALB/c mouse. 4T1 + PLT: coculture of the murine breast cancer cell line and the PLTs obtained from the BALB/c mouse.