Table 2.
Experimental information for DDI (genotoxic) and non-DDI (non-genotoxic) test chemicals used in this study.
| Test chemical | Chemical abbreviation | CAS No. | Chemical group# | Vehicle control | Concentrations tested (μm) |
|---|---|---|---|---|---|
| Aflatoxin B1 | AFB1 | 1162-65-8 | Group 1 | DMSO | 3.125, 6.25, 12.5, 15, 25 |
| Benzo[a]pyrene | BaP | 50-32-8 | Group 1 | DMSO | 0.9375, 1.875, 3.75, 7.5, 15 |
| Cisplatin | CISP | 15663-27-1 | Group 1 | DMSO | 3.125, 6.25, 12.5, 25, 50 |
| Cyclophosphamide | CP | 6055-19-2 | Group 1 | DMSO | 1,250, 2,500, 5,000, 7,500, 10,000 |
| Cytosine arabinoside | AraC | 147-94-4 | Group 1 | DMSO | 12.5, 25, 50, 100, 200 |
| Methyl methanesulfonate | MMS | 66-27-3 | Group 1 | DMSO | 22.7, 45.4, 90.8, 181.6, 363.2 |
| N-Nitroso-N-ethylurea | ENU | 759-73-9 | Group 1 | DMSO | 312.5, 625, 1,250, 2,500, 5,000* |
| Zidovudine (azidothymidine) | ZDV | 30516-87-1 | Group 1 | DMSO | 125, 250, 500, 1,000, 2,000 |
| Propyl gallate | PG | 121-79-9 | (Group 1) | DMSO | 125, 250, 500, 750, 1,000* |
| 2-Deoxy-D-glucose | 2DG | 154-17-6 | Group 2 | Water | 625, 1,250, 2,500, 5,000, 10,000 |
| Eugenol | EUG | 97-53-0 | Group 3 | DMSO | 156.25, 312.5, 625, 1,250, 2,500* |
| Urea | Urea | 57-13-6 | Group 3 | DMSO | 625, 1,250, 2,500, 5,000, 10,000 |
#
Chemical grouping based on Kirkland et al. (57, 58) recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests.
*
Indicates a cytotoxic concentration (<40% cell viability; >60% cytotoxic) that was subsequently eliminated from the gene expression analysis; all concentrations were used for CometChip® analysis.