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. 2021 Aug 18;13:701290. doi: 10.3389/fnsyn.2021.701290

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Copyright © 2021 Rodríguez-Manzo, González-Morales and Garduño-Gutiérrez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VTA NMDAR density does not change in response to sexual activity in male rats. Western blot analysis showing changes in the expression of the NMDAR constitutive GluN1 subunit in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of males that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, ^**P < 0.01. A comparison between the rats that copulated to satiety in the absence or presence of AM251 (Satiated + AM251) was conducted with the unpaired t-test, n.s. Values are mean ± S.E.M of the protein/β-Actin optical density (O.D.) ratios of 5 rats per group.