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. 2021 Aug 18;13:701290. doi: 10.3389/fnsyn.2021.701290

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Copyright © 2021 Rodríguez-Manzo, González-Morales and Garduño-Gutiérrez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Involvement of eCBs in the changes in pERK 1/2 expression in the VTA of sexually satiated rats. Western blot analysis showing changes in pERK1/2 expression in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of rats that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA, n.s. A comparison between the rats that copulated to satiety in the absence or presence of AM251 (Satiated + AM251) was conducted with the unpaired t-test, ^*P < 0.05. Values are mean ± S.E.M of the protein/β-Actin optical density (O.D.) ratios of 5 rats per group.