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. 2021 Aug 18;12:704112. doi: 10.3389/fphar.2021.704112

FIGURE 6.

FIGURE 6

SIRT1 deficiency impairs the effect of metformin on regulating cholesterol catabolism. Metformin reduced (A) hepatic cholesterol content in DIO mice (p < 0.05). (B) The relative mRNA level of CYP7B1 in the liver of DIO mice was reduced by metformin (p < 0.05). (C,D) The relative protein expression of CH25H was significantly upregulated (p < 0.05) but that of CYP7B1 was unaffected (p > 0.05) by metformin in the liver of DIO mice. Although (E) metformin also reduced hepatic cholesterol content (p < 0.05) and (F) downregulated the mRNA expression of CYP7B1 in Sirt1 +/− mice (p < 0.05), (G,H) the protein levels of CH25H were unaffected by metformin in the liver of Sirt11 +/− mice (p > 0.05). In C57BL/6J mice, *p < 0.05, HFD group vs Chow diet group, # p < 0.05, HFD + Metformin vs. HFD group. In Sirt1 +/− mice, *p < 0.05, WT + HFD + Metformin or Sirt1 +/− + HFD vs. WT + HFD group, # p < 0.05, Sirt1 +/− + HFD + Metformin vs. Sirt1 +/− + HFD group.