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Interference with TGR5 mRNA expression reduces Caco-2 monolayer epithelium barrier function. (A) The TEER of the cell monolayer of caco-2, n = 3. (B) The FITC-dextran permeability of the cell monolayer of Caco-2, n = 3. (C,D) Protein expression of TGR5 and Claudin-1 relative to β-actin. The results were confirmed by three independent experiments. Differences in data in cell subjects were assessed by one-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001.
