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. 2021 Aug 18;11:643039. doi: 10.3389/fonc.2021.643039

Figure 2.

Figure 2

The effect of LINC00284 silencing on the malignant activities of thyroid cancer cells. (A) The expression of LINC00284 in HTh74 cells with different treatments was determined using RT-qPCR. (B) The viability of HTh74 cells with different treatments was determined using the MTT assay. (C) The ALDH activity of HTh74 cells with different treatments was determined using the ALDEFLUOR assay. (D) Flow cytometry analysis of the cell cycle of HTh74 cells with different treatments. (E) Apoptosis of HTh74 cells with different treatments was determined using flow cytometry. (F) The colony formation ability of HTh74 cells with different treatments. (G) Self-renewal ability of HTh74 cells with different treatments was assessed using cell sphere formation assay. (H) The invasive ability of HTh74 cells with different treatments was assessed using Transwell assay. *p < 0.05 compared with the cells transfected with sh-NC. Data are presented as the means ± standard deviations. Comparisons between two groups were analyzed using the unpaired t-test, and comparisons among groups at different time points were analyzed using repeated measurement ANOVA with Tukey’s post hoc test. The experiments were repeated three times.