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. 2021 Aug 18;11:643039. doi: 10.3389/fonc.2021.643039

Figure 4.

Figure 4

The effect of the LINC00284/miR-30d-5p/ADAM12 axis on the biological activity of thyroid cancer cells. (A) The expression of LINC00284, miR-30d-5p and ADAM12 mRNA in C643 cells was determined using RT-qPCR. (B) The viability of C643 cells was determined using the MTT assay. (C) The ALDH activity of C643 cells was determined using the ALDEFLUOR experiment. (D) Flow cytometry analysis of the cell cycle of C643 cells d. (E) The apoptosis of C643 cells was determined using flow cytometry. (F) The colony formation ability of C643 cells. (G) Self-renewal ability of C643 cells assessed using the cell sphere formation assay. (H) The invasive ability of C643 cells assessed using the Transwell assay. *p < 0.05 compared with cells transfected with sh-NC. Data are presented as the means ± standard deviations. Comparisons between two groups were analyzed using the unpaired t-test, comparisons among multiple groups were analyzed using one-way ANOVA, followed by Tukey’s post hoc test, and comparisons among groups at different time points were analyzed using repeated measures ANOVA with Tukey’s post hoc test. The experiments were repeated 3 times. # p < 0.05 compared with cells transfected with miR-30d-5p mimic + oe-NC.