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. 2021 Aug 18;11:643039. doi: 10.3389/fonc.2021.643039

Figure 5.

Figure 5

The effect of the competitive binding of LINC00284 to miR-30d-5p to regulate the ADAM12/Notch axis on the biological activity of thyroid cancer cells. LINC00284 competitively binds to miR-30d-5p to upregulate the expression of ADAM12 and activate the Notch signaling pathway. (A) The expression of Notch1 and HES1 proteins in HTh74 cells was measured using Western blot analysis, *p < 0.05 compared with cells transfected with sh-NC. (B) The expression of Notch1 and HES1 proteins in C643 cells was measured using Western blot analysis, *p < 0.05 compared with cells transfected with sh-NC or NC mimic, # p < 0.05 compared with cells transfected with miR-30d-5p mimic + oe-NC. (C) The expression of Notch1 and HES1 proteins in HTh74 cells was measured using Western blot analysis. (D) The viability of HTh74 cells was determined using the MTT assay. (E) The ALDH activity of HTh74 cells determined in the ALDEFLUOR experiment. (F) Flow cytometry analysis of the cell cycle of HTh74 cells. (G) Flow cytometry analysis of the apoptosis of HTh74 cells. (H) The colony formation ability of C643 cells. (I) Self-renewal ability of C643 cells assessed using the cell sphere formation assay. (J) The invasive ability of C643 cells assessed using the Transwell assay. *p < 0.05 compared with cells treated with sh-LINC00284 + DMSO. Measurement data are presented as the means ± standard deviations. Comparisons between two groups were analyzed using the unpaired t-test, comparisons among multiple groups were analyzed using one-way ANOVA followed by Tukey’s post hoc test, and comparisons among groups at different time points were analyzed using repeated measures ANOVA with Tukey’s post hoc test. The experiments were repeated three times.