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. 2021 Aug 31;12(18):1787–1801. doi: 10.18632/oncotarget.28040

Figure 4. 5Z7O induces activation of apoptosis pathways and disrupts cell cycle progression.

Figure 4

(A) Supervised hierarchical clustering analysis of reverse-phase protein array (RPPA) performed in T-ALL cell lines treated with 3 μM 5Z7O for 21 hours. (B) Detection of proteins involved in apoptosis and cell cycle regulation in a panel of T-ALL and non-leukemic LCL cell lines after 5Z7O treatment as described in (A). (C) Immunofluorescence detection of phosphorylated H2AX (Alexa Fluor 488) in nuclei KOPT-K1, P12-Ichikawa, and RPMI8402 cells following 5Z7O treatment. DAPI was used to detect nuclei. Scale bar: 10 μm. (D) Diagram of DNA damage and G2/M checkpoint. Downregulated proteins upon 5Z7O treatment are indicated in blue.