Mice were SQ seeded with B16/F10 cells as previously and subsequently treated with DFMO/α-PD-1 therapy as previously described. Beginning on day 5 and continuing throughout the experiment, groups of mice were also given twice-weekly intraperitoneal injections of the indicated immune cell depletion antibodies (α-CD4, α-CD8, α-NK1.1, or α-GR1) (n=10/group) twice weekly. (A) Average growth of tumors within each cohort over time. (B) Area of individual tumors 19 days after tumor implantation (the day the first mock tumor reached predetermined end-point criteria). Statistical significance was determined using unpaired Student’s t-test (*=p<0.05, ** = p<0.005).