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. 2021 Aug 26;17(13):3672–3688. doi: 10.7150/ijbs.60110

Figure 3.

Figure 3

EndoA2 attenuates ischemia-induced cardiomyocyte apoptosis in MI mice. (A) TUNEL staining (red) on transverse sections of mouse hearts injected with Ad-EndoA2 or Ad-lacZ after MI. Scale bars: 100 µm (n=5 mice). (B) Quantification of TUNEL-positive cardiomyocytes. Injection of Ad-EndoA2 significantly decreased cardiomyocyte apoptosis induced by LAD ligation (n=5 mice, ** p <0.01 vs. sham; ## p <0.01 vs. MI+Ad-lacZ). (C-D) Western blotting results showed that Ad-EndoA2 inhibited cardiomyocyte apoptosis in the infarct border zone of ischemic hearts, which decreased the expression of cleaved caspase-3, Bax and increased the ratio of Bcl-2/Bax and Bcl-2 expression compared with MI+Ad-lacZ group (n=5, ** p <0.01 vs. sham; ## p <0.01 vs. MI+Ad-lacZ).