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. 2021 Aug 19;17(13):3583–3594. doi: 10.7150/ijbs.61647

Figure 6.

Figure 6

The knockdown of Arkadia increases Smad7 and abolishes the effect of recombinant latent TGF-β1 protein on high glucose induced fibrosis and inflammation in cells. (A) Knockdown of Arkadia in mesangial cells. (B) The level of Smad7 increased in mouse mesangial cells with Arkadia knockdown. (C) The knockdown of Arkadia in high glucose treated cells abolished the effect of latent TGF-β1. LG, low glucose (5.5 mM D-glucose). HG, high glucose (35 mM D-glucose). LT1, recombinant latent TGF-β1 protein (20 ng/ml). EV, Empty vector. KD, Knockdown. Data represent the means ± SEM from 3-4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus EV-LG control; #P < 0.05, ##P < 0.01, ###P < 0.001 versus EV-HG treatment. $P < 0.05, $$ P < 0.01, $$$ P < 0.001 versus KD-LG control.