Figure 4.

TQ blocks autophagic flux in 5637 and T24 cells. (A) 5637 and T24 cell lines stably expressing RFP-GFP-LC3B protein were established using lentivirus transfection, then treated with different administration, including CQ (10 μM, 6 h), EBSS (12 h), TQ (50 μM, 24 h), CQ pretreatment (10 μM, 6 h) with TQ (50 μM, 24 h) and EBSS pretreatment (12 h) with TQ (50 μM, 24 h). Confocal microscope was used to observe the autophagic flux alterations of BC cells under different treatment. (B) Quantification analysis of autophagosomes and autolysosomes of cancer cells in Figure 4A. Data are shown as the mean ± standard deviation (SD). CON: control; CQ: Chloroquine; EBSS: Earle's balanced salt solution; *p<0.05, **p<0.01.