Figure 5.

TQ induced Impaired autophagic flux mediates the cell apoptosis of BC. (A) 5637 and T24 cells were administrated with TQ of 50 μM for 24 h or not, transmission electronical microscopy was used to observe the autophagosomes formation in two cell lines. (B, C) TQ treated (50 μM, 24 h) 5637 and T24 cells were pre-administrated with CQ (10 μM, 6 h) or EBSS (12 h), followed by WB assays detecting the expression levels of apoptosis related proteins (cleaved PARP, cleaved caspase 3 and Bcl-2) and autophagic markers (LC3B and p62). (D, E) CCK8 assay was conducted to test the cell viability of BC cells same treated as Figure 5B and 5C. (F) BC cells were pre-administrated with control medium or the following administrations: NAC (5 mM, 4 h), CQ (10 μM, 6 h), EBSS (12 h) and ZVF (20 μM), and then exposed to TQ of 50 μM for 24 h. Flow cytometry assay was conducted to test the apoptosis rate of cancer cells co-stained with PI and FITC. ZVF: Z-VAD-FMK; WB: western blot; *p<0.05, **p<0.01.