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. 2021 Aug 19;12:716654. doi: 10.3389/fphys.2021.716654

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Copyright © 2021 Chen, Wang, Gao, Ma, Xue and Hao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LEfSE analysis and PICRUSt analysis results. (A) The LEfSe cladogram in green shows the taxa enriched in the T group, and that in red shows the taxa enriched in the C group. The diameter of nodes is directly proportional to the relative abundance. (B) Taxonomic cladogram obtained using linear discriminant analysis (LDA) effect size (LEfSe) analysis and Mann–Whitney U-tests of the 16S sequences. LEfSe identified the taxa with the greatest differences in abundance between the T group and C group. At the phylum, class, order, family and genus levels, the control-enriched taxa are indicated by a positive LDA score (red), and T group-enriched taxa are indicated by a negative score (green). Only taxa meeting a significant LDA threshold value of > 2 are shown. (C) PICRUSt analysis results of predicted functional pathways in the gut microbiota.