Table 3.
Data acquisition | ||||
Instrument | SWING beamline (SOLEIL, France) | |||
Detector | Dectris EIGER 4 M | |||
Wavelength (Å) | 1.03324 | |||
Energy (keV) | 12 | |||
Sample to detector distance (m) | 2.0 | |||
s-range (Å−1) | 0.0041 – 0.5597 | |||
SEC-SAXS experiments | ||||
ED-1 | ED-2 | ED-3 | ED-4 | |
SEC column | Superdex S200 Increase 5/150 GL (GE Healthcare) | |||
Injection volume (µl) | 50 | |||
Protein concentration (mg.ml−1) | 7.7 | 5.0 | 14.6 | 8.3 |
Flow rate (ml.min−1) | 0.2 | |||
Temperature (°C) | 20 | |||
Exposure | Continuous: 1 frame/s (990 ms exposure and 10 ms dead time) | |||
Buffer | HBS (10 mM HEPES, 150 mM NaCl pH 7.4) | |||
smin (Å -1) | 0.0114020 | 0.00821002 | 0.00961379 | 0.00912193 |
I(0) (cm−1) | 0.0430 | 0.02892 | 0.04588 | 0.02070 |
± 1.8 x10-4 | ± 0.94 x10-4 | ± 3.24 x10-4 | ± 1.42 x10-4 | |
Guinier analysis smax.Rg / number of points | 1.29 / 23 | 0.998 / 34 | 1.28 / 24 | 0.985 / 33 |