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. 2021 Jul 19;12:100081. doi: 10.1016/j.mbplus.2021.100081

Table 3.

Experimental setup for the collection of SEC-SAXS scattering intensities.

Data acquisition
Instrument SWING beamline (SOLEIL, France)
Detector Dectris EIGER 4 M
Wavelength (Å) 1.03324
Energy (keV) 12
Sample to detector distance (m) 2.0
s-range (Å−1) 0.0041 – 0.5597



SEC-SAXS experiments
ED-1 ED-2 ED-3 ED-4

SEC column Superdex S200 Increase 5/150 GL (GE Healthcare)
Injection volume (µl) 50
Protein concentration (mg.ml−1) 7.7 5.0 14.6 8.3
Flow rate (ml.min−1) 0.2
Temperature (°C) 20
Exposure Continuous: 1 frame/s (990 ms exposure and 10 ms dead time)
Buffer HBS (10 mM HEPES, 150 mM NaCl pH 7.4)
smin-1) 0.0114020 0.00821002 0.00961379 0.00912193
I(0) (cm−1) 0.0430 0.02892 0.04588 0.02070
± 1.8 x10-4 ± 0.94 x10-4 ± 3.24 x10-4 ± 1.42 x10-4
Guinier analysis smax.Rg / number of points 1.29 / 23 0.998 / 34 1.28 / 24 0.985 / 33