Figure 5.

Identification of putative downstream genes of Loc100506691 in gastric cancer by applying the microarray approach

(A) Differential expression genes were identified in HR with Loc100506691 overexpression and TSGH with Loc100506691 knockdown and compared with the corresponding control using a microarray approach. The heatmap presents putative downstream targets of Loc100506691in gastric cancer cells. (B) Expression levels of CHAC1 were examined by real-time PCR in TSGH and HR cells with Loc100506691 knockdown. (C) Expression levels of CHAC1 were assessed in HR cells with pLoc100506691-V1 or -V2 overexpression by applying the real-time PCR approach. (D) Expression levels of CHAC1 were examined by western blotting in gastric cancer cells with Loc100506691 knockdown or overexpression in TSGH and HR, respectively. Relative expression was further quantification (below panels). (E) The CHAC1 expression levels in human gastric cancer cells were examined through western blotting. Relative expression was further quantification (below panel). (F and G) After transfection for 24 h, CHAC1 expression levels were examined in TSGH with siCHAC1 using western blotting and real-time PCR. (H) Cell proliferation was examined in TSGH with CHAC1 knockdown. (I and J) The colony-formation ability of TSGH was investigated and quantified following siCHAC1 transfection. (K and L) The migration and invasion ability were assessed and quantified in TSGH with CHAC1 knockdown (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).