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. 2021 Jul 21;26:333–346. doi: 10.1016/j.omtn.2021.07.007

Figure 8.

Figure 8

Knockin of Mettl3 promotes in vivo ESCC tumorigenesis

(A) Experimental design for inducing mouse ESCC by 4NQO with or without epithelium-specific Mettl3 knockin. (B) Western blot with METTL3 antibody. (C) Representative image of esophageal lesions 20 weeks after 4NQO treatment. (D) Quantification of lesion areas in control group and cKI group. (E) Representative H&E staining of esophagus tissues in control group and cKI group. (F and G) Quantification of dysplasia numbers (F) and ESCC numbers (G) in esophagus with or without Mettl3 cKI. (H) Representative images of 3METTL3 and Ki67 IHC staining in esophageal tissues with or without Mettl3 cKI. (I) Quantification ofMETTL3 and Ki67 staining scores in control group and cKI group. (J) Representative images of NOTCH1 and HES1 IHC staining in control group and cKI group. (K) Quantification of NOTCH1 and HES1 staining scores in control group and cKI group. Ctrl, control. Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).