Figure 7.

Effect of miR-1207-5p on tumor growth in a xenograft model

Nude mice were subcutaneously administered with TU-177 cells to construct a xenograft model. After tumor formation, miR-1207-5p agomir or negative control (NC) agomir was injected intratumor. (A) Representative images and growth curve of tumor at 12 days after injection of miR-1207-5p agomir or NC agomir in LSCC xenograft model. (B) The xenograft tumor weight was measured and plotted at 12 days after injection. (C) miR-1207-5p expression in xenograft tumors validated by qPCR. (D) The expression changes of SKA3, Ki-67, E-cadherin, N-cadherin, Snail, and Vimentin in xenograft tumors were examined by IHC staining. Representative images are shown. Scale bars, 25 μm. ∗p < 0.05 and ∗∗p < 0.01. Data are mean ± SD of three separate experiments. (E) Schematic model depicting that miR-1207-5p/SKA3 signaling axis regulates LSCC progression.