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. 2021 Aug 19;9:716501. doi: 10.3389/fcell.2021.716501

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Copyright © 2021 Wang, Wu, Gao, Li, Peng, Fan, Xie, Guo and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GADD45B predicted a good prognosis and had no effect on the proliferation and migration of PCa cells. (A) Representative immunohistochemical staining and quantification showed the expression level of GADD45B in PCa tissues of patients from Sun Yat-sen University Cancer Center. Magnification 100×, scale bar = 200 μm, Magnification 400×, scale bar = 100 μm. (B) Biochemical recurrence survival analysis of GADD45B in patients form Sun Yat-sen University Cancer Center. (C,D) RT-PCR and WB analysis of GADD45B levels in GADD45B-overexpressing DU145 cell line. (E) The MTS assay test of cell viability of DU145 cells with GADD45B overexpression. (F,G) Representative images and histogram analysis of migration assays after upregulation of GADD45B. ***p < 0.001 and not statistically significant p > 0.05.