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. 2021 Sep 3;12(9):827. doi: 10.1038/s41419-021-04107-7

Fig. 6. Inhibition of pSrc and pFAK reverse the promotion effect of PPFIBP1 on the migration and invasion.

Fig. 6

A, B Western blots showing the indicated proteins of U251 MG cells treated with or without 50 nM Dasatinib (A) or 1 μM PF562711 (B) for 30 min. The numbers represent the expression of pY397 FAK, normalized to FAK, and the expression of pY416 Src, normalized to Src. The figures are representative data from at least three independent experiments. C, D Wound-healing assays of U251 MG derived cells with PP-OE and the Ctrl at indicated time points. Left: representative bright-field pictures of cells at 0 or 20 h. Right: relative quantification of the (20 h/0 h) wound width. Dashed line represents the wound edge. Cells were treated with or without Dasatinib (C) or PF562711 (D) after scratches. E Transwell invasion assay with PP-OE and the Ctrl U251 MG cells. Left: Representative images of U251 MG cells stained with crystal violet. Cells were treated with or without 50 nM Dasatinib or 1 μM PF562711. Right: number of invaded cells through the membrane per field. The figures are representative data from at least three independent experiments. The data represents the mean ± SEM (n ≥ 3). Statistical analysis was performed using Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.