Table A1.
Sex-based differences in ion channel subunit expression from non-diseased human ventricles.
| Ion channel | Gene/protein | Epi | Endo | Mid | ||||
|---|---|---|---|---|---|---|---|---|
| male | female | male | female | interp | male | female | ||
| I NaL | NaV1.5 | 310.6 | 260.8 | 426.8 | 373.8 | 403.5 | ||
| Ratio | 0.77 | 0.65 | 1.06 | 0.93 | 1.06 | 0.93 | ||
| Based on ΔNaV1.5 expression (O'Hara et al., 2011). | ||||||||
| I pCa | Ca2+ ATPase 4 | 377.0 | 682.1 | 426.8 | 685.2 | 540.5 | ||
| Ratio | 0.70 | 1.26 | 0.79 | 1.27 | 1.97 | 3.17 | ||
| Based on ΔCa2+ ATPase 4 expression (Yang and Clancy, 2012). | ||||||||
| I Kr | hERG | 179.5 | 144.2 | 164.8 | 130.5 | 149.7 | ||
| Ratio | 1.20 | 0.96 | 1.10 | 0.87 | 0.88 | 0.70 | ||
| Based on ΔhERG expression (O'Hara et al., 2011). | ||||||||
| I Ks | MinK | 13.6 | 7.3 | 11.9 | 5.8 | 9.2 | ||
| Ratio | 1.16 | 0.93 | 1.10 | 0.88 | 1.10 | 0.88 | ||
| Based on 1/3 Δ MinK expression (Yang and Clancy, 2012). | ||||||||
| I K1 | Kir2.3 | 91.2 | 21.4 | 92.7 | 55.2 | 76.2 | ||
| Ratio | 1.07 | 0.76 | 1.07 | 0.91 | 1.39 | 1.18 | ||
| Based on 1/3 Δ Kir2.3 expression (Yang and Clancy, 2012). | ||||||||
| I NaCa, i/ss | NCX1 | 821.1 | 801.4 | 754.5 | 739 | 747.7 | ||
| Ratio | 1.10 | 1.07 | 1.01 | 0.99 | 1.41 | 1.38 | ||
| Based on Δ NCX1 expression (O'Hara et al., 2011). | ||||||||
| I NaK | Na+/K+ ATPase α1 | 207.7 | 513.4 | 269 | 622.5 | 424.54 | ||
| Na+/K+ ATPase α3 | 1481 | 917.8 | 1547.6 | 1014.2 | 1312.904 | |||
| Ratio | 0.92 | 0.87 | 1.00 | 1.00 | 0.70 | 0.70 | ||
| Based on 1/3 ΔNa+/K+ ATPase α1 and 2/3 ΔNa+/K+ ATPase α3 expression (Yang and Clancy, 2012). | ||||||||
| I Kb | KV1.5 | 12.7 | 6.5 | 19.5 | 10.5 | 15.54 | ||
| Ratio | 0.82 | 0.42 | 1.25 | 0.68 | 1.25 | 0.68 | ||
| Based on ΔKV1.5 expression (O'Hara et al., 2011). | ||||||||
| Ca2+ release | RYR2 | 6213.7 | 4890.6 | 5463.9 | 5582.5 | 5516.084 | ||
| Ratio | 1.13 | 0.89 | 0.99 | 1.01 | 1.68 | 1.72 | ||
| Based on ΔRYR2 expression (O'Hara et al., 2011). | ||||||||
| Ca2+ uptake | SERCA2 | 4850.5 | 6728.4 | 3410.4 | 3921.9 | 3635.46 | ||
| Ratio | 1.33 | 1.85 | 0.94 | 1.08 | 0.94 | 1.08 | ||
| Based on ΔSERCA2 expression (Yang and Clancy, 2012). | ||||||||
| [CMDN] | CALM3 | 1326.9 | 1955.5 | 1206.9 | 1600.5 | 1380.084 | ||
| Ratio | 0.97 | 1.28 | 0.92 | 1.11 | 0.92 | 1.11 | ||
| Based on 2/3 Δ CALM3 expression (Yang and Clancy, 2012). | ||||||||
Overview of the sex differences in ion channel subunit expression for the channels used in our cell-scale and multi-scale computational models. The gene/protein data represents the normalized relative expression (2-ΔCt) deduced from Gaborit et al. (2010). The endocardial interp column represents the relative ion channel subunit expression for the hypothesized 56% male, 44% female model that the original endocardial O'Hara Rudy cell model was based on. To compute the individual ion channel activity scalings, we followed scaling rules established by O'Hara et al. (2011) and Yang and Clancy (2012), as reported below each set of scaling ratios.