Figure 7.

Ethidium bromide stained agarose gels show size of products from PCR and subcloning lipocalin-interacting membrane receptor. (A) Shows RT-PCR products from corneal epithelium. (B) Shows plasmid inserts from single colonies from subcloning of RT- PCR of conjunctiva. (C) Shows plasmid inserts from single colonies from subcloning the PCR products of the corneal epithelial library. Lanes 2, 6, and 13 show 100 base pair molecular weight markers, arrowheads show the first marker at 100 base pairs. Target products lane 1 keratin 14 (control for cornea) using forward primer 5′-AGCCGCATTCTGAACGAGAT-3′ and reverse primer 5′-TCGTGCACATCCATGACCTT-3′, expected product size 529 bases; lanes 3, 5–10 show lipocalin-interacting membrane receptor using forward primer 5′-GTGCTTGCTGGTGCTGACGG-3′ and reverse primer 5′-TCACTGGTGCTGGGTCTTCCTAGATG-3′). Lane 3 uses plasmid with cloned lipocalin receptor as a positive control. Lane 4 is negative control (absence of template). The two sized products (e.g., Lane 5) were shown by sequencing to represent isoform, one which lacks a 60 base pair sequence corresponding to amino acids missing from isoform 3, Q6UX01-3, an alternative splice variant (as shown in Figure 3) (https://www.uniprot.org/uniprot/Q6UX01).