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. 2021 Aug 19;12:635701. doi: 10.3389/fimmu.2021.635701

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Copyright © 2021 Peroni, Toscaro, Canateli, Tonoli, de Olivera, Benedetti, Coimbra, Pereira, Marques, Proença-Modena, Lima, Viana, Borges, Lin-Wang, Abboud, Gun, Franchini and Bajgelman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ELISA assay for detection of mice antiviral antibodies. Mice were immunized with inactivated SARS-CoV-2 virus, and serum samples were collected with 14 days. (A) Plates were coated with 1.0 mg/ml of indicated viral antigens or 1:250 of inactivated virus. (B) plates were adsorbed with 100 ng/ml of indicated viral antigens or 1:250 of inactivated virus. Target antigens: prot.N (N protein), prot.3CL (3CL protein), prot.S (S protein), inactivated virus 1:250 (inactivated virus diluted 1:250), PBS 1x (1X PBS diluent as negative control). Two-way ANOVA, p < 0.05 for IS against NIS for all target antigens, but PBS 1x, in all dilutions. Representative experiment of two independent experiments. The serum of five animals was pooled for each condition.