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. 2021 Aug 19;12:635701. doi: 10.3389/fimmu.2021.635701

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Copyright © 2021 Peroni, Toscaro, Canateli, Tonoli, de Olivera, Benedetti, Coimbra, Pereira, Marques, Proença-Modena, Lima, Viana, Borges, Lin-Wang, Abboud, Gun, Franchini and Bajgelman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ELISA assay for IgG detection in human samples. (A) Serum samples diluted 1:100 were incubated to N protein-coated ELISA plates. (B) Serum samples diluted 1:100 were incubated to 3CL protein-coated ELISA plates. (−) negative control, absence of serum; NIS: non-immune human serum. the PCR status of nasal and oropharyngeal swab related to serum donors is indicated below the graph. Cutoff was set to NIS × 1.3. Representative experiment of two independent experiments.