FIGURE 8.

ZmWRKY83 overexpression in transgenic Arabidopsis increases B. cinerea resistance. (A) The relative expression level of ZmWRKY83 in Col-0 and ZmWRKY83 overexpressing transgenic plants. (B) Altered disease resistance of Col-0, atwrky33 mutant, and ZmWRKY83 overexpressing transgenic plants against B. cinerea. Four-week-old plants were inoculated by B. cinerea spore suspension (10⁶ ml^–1) and kept at high humidity. The experiments were repeated three times with similar results. (C) Measurement of lesion areas on B. cinerea-inoculated Arabidopsis leaves shown in panel B. The necrotic lesion areas on each leaf were measured using ImageJ. Data represent the mean ± SD. (D) The relative expression level of ZmWRKY83 in B73 and zmwrky83 mutant. (E) Altered disease resistance of B73, zmwrky33 mutant against F. graminearum. V7 stage plants were inoculated by F. graminearum spore suspension (10⁶ ml^–1) and kept at high humidity. (F) Measurement of lesion length on F. graminearum-inoculated maize stem shown in panel E. Data represent the mean ± SD.