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. 2021 Aug 27;53(8):1238–1249. doi: 10.1038/s12276-021-00661-4

Fig. 4. P62 deficiency leads to downregulated LH and oxidative phosphorylation (OXPHOS) in pituitary gonadotropin cells.

Fig. 4

a–c In vitro experiments in which the mouse pituitary gonadotroph LβT2 cell line was transfected with control/p62 siRNA for 48 h. a RT-PCR detection of p62, Lhb, Fshb, and GnRHR mRNA, n = 4–6. b WB detection of silenced p62 expression and LH protein levels. c Immunofluorescence detection of LH (green) and FSH (red) protein expression in LβT2 cells. DAPI in blue, scale bar: 20 μm. d The significant changed pathways in p62−/− mouse pituitaries enriched by KEGG analysis. e Differentially expressed pituitary OXPHOS markers between p62−/− and p62+/+ mice shown as a heatmap from RNA-Seq of young female mice, n = 3. f Relative mRNA expression levels of OXPHOS markers (mitochondrial complex I: Ndufa1–4; complex II: Sdhb; complex III: Uqcrq, Uqcrh, Uqcr11; complex IV: cox4i1, cox7a1, cox7a2; complex V: atp5c1, atp5k) with p62 siRNA treatment, n = 4. g NADH, NAD+ concentration and NAD+ /NADH ratio measurement, n = 4. All data are presented as the mean ± SD. Student’s t-test, *P < 0.05; **P < 0.01; ***P < 0.001.