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. 2021 Sep 3;11:17632. doi: 10.1038/s41598-021-96778-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed genes (DEGs) in RK-fed, cuelure non-responding and RK-unfed, cuelure responding males. (a) Principal component analysis shows that colour-coded replicates show separation between conditions. (b) The expression profile of individual samples was explored with mean-difference (MD) plots. The differentially expressed genes are highlighted in red. The blue guidelines mark the log fold changes between ± 2. (c) Heatmap of all DEGs from all samples, with unsupervised hierarchical clustering on X and Y axes; drawn with coolmap function [https://rdrr.io/bioc/limma/man/coolmap.html] from bioconductor limma (3.48.1) package⁵⁹. Clustering of samples was evident, as was clustering of genes that had correlated expression patterns.