Figure 3.

ANGPTL4 and ANGPTL4/8 inhibition of recombinant EL protein activity. The ability of ANGPTL4 or the ANGPTL4/8 complex to inhibit human recombinant EL protein activity was assessed using phospholipase A₁ selective substrate. Fluorescence was monitored with an excitation wavelength of 485 nm and an emission wavelength of 516 nm. Readings were taken at 1 and 30 min, with the 1-minute reading subtracted from the 30-minute reading to correct for background fluorescence. Results are shown as the mean ± SD (n = 4 from 2 independent experiments).