Table 1.
BEMAP analysis of glycosylated YghJ. YghJ was digested with Trypsin and O-linked glycosylated peptide sequences were identified using BEMAP in combination with mass spectrometry.
| start | end | Sequence | Site# | Site# | Site# | Site# | Site# | Site# | Site# |
|---|---|---|---|---|---|---|---|---|---|
| 91 | 101 | TGYLTLGGsQR | 99 | ||||||
| 102 | 143 | VtGAtCNGEssDGFtFKPGEDVtCVAGNtTIATFNTQSEAAR | 103 | 106 | 111 | 112 | 116 | 124 | 130 |
| 169 | 199 | SNAVSLVTsSNsCPANtEQVCLtFSSVIESK | 177 | 180 | 185 | 191 | |||
| 218 | 231 | LVNEEVENNAAtDK | 229 | ||||||
| 355 | 364 | YsttGQNNtR | 356 | 357 | 358 | 363 | |||
| 419 | 435 | EIDtAICAKTDGCNEAR | 422 | ||||||
| 428 | 442 | tDGCNEARWFsLttR | 428 | 438 | 440 | 441 | |||
| 456 | 468 | LWGVDTNYKSVsK | 467 | ||||||
| 469 | 486 | FHVFHDStNFYGsTGNAR | 476 | 481 | |||||
| 487 | 504 | GQAVVNIsNAAFPILMAR | 494 | ||||||
| 588 | 609 | DGQCtLNsDPDDMKNFMENVLR | 592 | 595 | |||||
| 610 | 615 | YLsnDR | 612 | ||||||
| 622 | 636 | ssMtVGTNLEtVYFK | 622 | 623 | 625 | 632 | |||
| 720 | 734 | GGsVLIMENVMSNLK | 722 | ||||||
| 735 | 743 | EEsAsGFVR | 739 | ||||||
| 757 | 769 | sVVnNDPQGYPDR | 757 | ||||||
| 818 | 830 | LEVAsWQEEVEGK | 822 | ||||||
| 845 | 853 | TPEsLAAAK | 848 | ||||||
| 909 | 922 | AMLQAADLGtNIQR | 918 | ||||||
| 923 | 935 | LYQHELYFRtNGR | 932 | ||||||
| 1000 | 1015 | KsLIDNKMIYGEEssK | 1001 | 1013 | 1014 | ||||
| 1016 | 1034 | AGMMNPsYPLNYMEKPLTR | 1022 | ||||||
| 1048 | 1075 | VDVEKYPGVVNtNGEtVtQNINLYSAPTK | 1059 | 1063 | 1065 | ||||
| 1101 | 1120 | StVPVtVTVALADDLtGREK | 1102 | 1106 | 1116 | ||||
| 1134 | 1142 | tYDLKANDK | 1134 | ||||||
| 1134 | 1146 | TYDLKANDKVtFK | 1144 | ||||||
| 1341 | 1362 | VADDITVAPEYLEEsNGQAWAR | 1355 | ||||||
| 1418 | 1431 | ARGDEVsNDKFGGK | 1424 |
The start and end position of each of the 28 peptide sequences within YghJ are listed, as well as the specific residue number which is modified. Lower case s or t indicate modified residue.