Figure 3.
The migration dynamics of RABV in mice post i.m. inoculation with a recombinant RABV expressing teLuc. (A) Schematic diagram showing the construction of recombinant RABV expressing teLuc, termed RABV-teLuc. (B) Single-step growth curves of RABV-teLuc and the parent virus in BSR cells infected at an MOI of 0.01. (C) Expression of teLuc in BSR cells infected with the indicated dose of RABV-teLuc at 24h post-infection (hpi). (D) Early detection of RABV-teLuc infection in a mouse model. Twelve 6-week-old BALB/c mice were randomly divided into four groups, with three mice in each group. Three groups of mice were i.m. inoculated with 50×mouse LD50 RABV-teLuc, and the mock group was i.m. inoculated with the same volume of DMEM. During 3–5 dpi, one of the three groups of RABV-infected mice was taken every other day for intrathecal injection with 0.3μmol diphenylterazine (DTZ) per mouse. The mock group was inoculated with 0.3μmol DTZ per mouse. The mice in each group were imaged by an IVIS Lumina III 5min post-injection with DTZ.