Figure 5.

Viral load in mouse brains post i.c. inoculation with AAVs expressing CR57 or EGFP. Groups of 6-week-old female BALB/c mice (n=10) were i.m. injected with 50×mouse LD₅₀ DRV and i.c. inoculated with 10¹² vector genome of AAVs at the 4 dpi. The mock group was inoculated with the same volume of DMEM at 4 dpi. At 12 dpi, the brains were collected, and half of the brain was fixed, paraffin-embedded, sectioned, and analyzed by immunohistochemistry by staining with antibodies against RABV-P (A). Scare bar, 50μm. The other half of the brain was analyzed for RABV-N levels by Western blotting (B).