Figure 3.

Microinjection

(A) Using the 40× objective, focus on the two rows of germline nuclei as shown and insert the needle into the pachytene zone. Flow of the injection mixtures should reach mature oocytes in the proximal end of the germline as shown with yellow dotted line.

(B) Snapshots from Methods Video S1 are shown to illustrate the flow of the injection mixture. Germline is shown (1) before and (2) after injection. Arrows marks the region in the gonad until where the mixture has reached. 10× magnification is shown for illustration purposes, use 40× objective for the microinjection procedure.