Thermocycling conditions:
| Step | Temperature | Time |
|---|---|---|
| Initial denaturation | 98°C | 1 min |
| Amplification (30 cycles) | 98°C | 10 s |
| 64°C | 20 s | |
| 72°C | 40 s | |
| Final extension | 72°C | 3 min |
| 4°C | Hold |
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