REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Hydrofluoric acid (HF)	Sigma-Aldrich	339261
Halocarbon oil- 700 CAS#9002-83-9	Sigma-Aldrich	H8898
Agarose	Genesee Scientific	20-102GP
tracrRNA	IDT	Cat# 1072532
TE 7.5 (10 mM Tris, 0.1 mM EDTA)	IDT	Cat#11-01-02-02
Nuclease-Free Duplex Buffer (30 mM HEPES, pH 7.5; 100 mM potassium acetate)	IDT	Cat#11-01-03-01
Polyethylene Glycol 8000 (PEG)	MP	#195445
Q5 polymerase	NEB	Cat# M0491S
S. pyogenes Cas9 3NLS protein	IDT	Cat#1081058
Cas12a protein	IDT	Cat#10001272
Critical commercial assays
Gel Extraction Kit	QIAGEN-Qiaquick	#28706
Experimental models: Organisms/Strains
C. elegans N2 strain	Caenorhabditis Genetics Center	N/A
Oligonucleotides
CRISPR-Cas9 crRNA, 2 nmol	IDT	N/A
A.s. Cas12a crRNA, 2 nmol	IDT	N/A
ssODN donors (ultramer)	IDT	N/A
GFP forward primer	This study	AGTAAAGGAGAAGAACTT
GFP reverse primer	This study	TTTGTATAGTTCATCCATGC
Universal linker forward	This study	TCCGGAGGGAGTGGA
Universal linker reverse	This study	AGAACCTCCGCCACC
Recombinant DNA
GFP-linker plasmid	Addgene	N/A
FLAG-TEV-degron-linker plasmid	Addgene	N/A
mCherry linker plasmid	Addgene	N/A
PRF4::rol-6 (su1006); now named pCCM958	Addgene	N/A
Other
Glass capillaries	World Precision Instruments	#1B120F-4
Cover slips 24 x 60 mm No.1	Globe Scientific Inc	#1419-10
Cover slips 22 x 22 mm No.1	Globe Scientific Inc	#1404-10
Mouth pipette -15 In Drummond aspirator tube assembly	Thermo Fisher Scientific	#2118010
PCR purification columns	QIAGEN-Minelute	#28604
Ampure XP beads	Beckman Coulter	Ref# A63880
Tygon tubing E-3603 (ID:1/32 in; OD:3/32 in; Wall: 1/32 in)	Saint-Gobain	#00444
Microloader Tips (Femtotips)	Eppendorf	930001007
Dissecting scope	Nikon	SMZ745
Inverted microscope (DIAPHOT 200)	Nikon	Current successor: Eclipse Ti2
Fluorescence dissecting microscope	Zeiss	Axio Zoom. V16
Needle Puller Narishige PN-30	Narishige	Current successor: PC-100
Microinjector	Tritech Research, Inc	Analog MINJ-1
Micromanipulator	Narishige	MN-151
Thermal Cycler	Bio-Rad	1851148