Table 1.

Real-time PCR primer sequences.

Gene ID	Sequence	GC%	Tm	length(bp)
Occludin	CCTTGTTGGCCATGTGCAG	57.9	63.7	78
	GGTCCACGGTGCAGTAGTGGTA	59.1	64.4
ZO-1	TGGCAATCAACTTTGGGTAGCA	45.5	64.8	156
	ATCCACAGAGGCAACTGAACCATA	45.8	64.1
Claudin-1	CTCCCAAGCAGCTGCATATCTC	54.5	63.5	148
	GCTCAGTCAGGCTAAGAACACCAA	50	64.1
β-actin	ATTGTCCACCGCAAATGCTTC	47.6	64.4	113
	AAATAAAGCCATGCCAATCTCGTC	41.7	64.5

Note: β-actin is an internal reference gene.