Figure 6.

Expression profiling of lignan biosynthesis pathway genes in five tissues of M. officinalis by RNA-Seq

(A) The general biosynthetic pathway of lignans. The solid arrows are reactions catalyzed by known enzymes, whereas the dashed arrows are the predicted lignan biosynthetic steps in M. officinalis.

(B) Expression values of lignan biosynthesis pathway genes. R, roots; F, fruits; S, stems; L, leaves; T, twigs; C4H, cinnamate 4-hydroxylase; COMT, caffeic acid 3-O-methyltransferase; CCOAMT, caffeoyl-CoA O-methyltransferase; HCT, shikimate O-hydroxycinnamoyltransferase; SIRD, secoisolariciresinol dehydrogenase; CAD, cinnamyl-alcohol dehydrogenase; CCR, cinnamoyl-CoA reductase; 4CL, 4-coumarate-CoA ligase; PLR, pinoresinol-lariciresinol reductase; PAL, phenylalanine ammonia-lyase.