Table 5.
Association of clinicopathological variables and individual markers with disease-free survival and overall survival in the second cohort in univariate and multivariate analyses.
| Variables | a. Univariate analysis | b. Multivariate analysis | c. Univariate analysis | d. Multivariate analysis | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HR | 95%CI | p value | HR | 95%CI | p value | HR | 95%CI | p value | HR | 95%CI | p value | ||
| Disease-free survival | Overall survival | ||||||||||||
| Sex | Woman vs Man | 0.819 | 0.430-1.512 | 0.5283 | 0.919 | 0.395-2.048 | 0.8320 | ||||||
| Location | Rectum vs Left colon | 1.982 | 0.976-4.345 | 0.0588 | 1.617 | 0.639-4.603 | 0.3180 | ||||||
| Right colon vs Left colon | 1.094 | 0.417-2.774 | 0.8505 | 1.088 | 0.313-3.613 | 0.8894 | |||||||
| Right colon vs Rectum | 1.813 | 0.850-4.310 | 0.1278 | 1.486 | 0.560-4.632 | 0.4393 | |||||||
| pT | pT4 vs pT3 | 2.279 | 1.142-4.307 | 0.0208 | 1.527 | 0.714-3.112 | 0.2662 | 2.630 | 1.065-5.986 | 0.0369 | 1.936 | 0.746-4.670 | 0.1667 |
| Stage | III vs II | 3.114 | 1.633-6.334 | 0.0004 | 1.991 | 0.977-4.301 | 0.0581 | 4.599 | 1.847-13.885 | 0.0007 | 2.619 | 0.980-8.321 | 0.0552 |
| Histological type | WDA vs MDA | 0.627 | 0.264-1.492 | 0.2657 | 0.787 | 0.269-2.303 | 0.6533 | ||||||
| Lymphatic invasion | Positive vs Negative | 4.334 | 0.944-76.833 | 0.0620 | 2.447 | 0.516-43.777 | 0.3125 | ||||||
| Venous invasion | Positive vs Negative | 4.855 | 1.057-86.076 | 0.0401 | 4.073 | 0.838-73.423 | 0.0904 | 2.722 | 0.574-48.690 | 0.2501 | |||
| Tumor budding | High vs Low | 2.291 | 1.170-4.285 | 0.0169 | 1.667 | 0.825-3.237 | 0.1497 | 2.090 | 0.847-4.752 | 0.1052 | |||
| Desmoplastic reaction | Immature vs Intermediate | 1.520 | 0.712-3.204 | 0.2736 | 1.227 | 0.451-3.118 | 0.6754 | ||||||
| Immature vs Mature | 2.322 | 1.076-4.976 | 0.0324 | 0.993 | 0.415-2.340 | 0.9867 | 3.293 | 1.092-10.245 | 0.0348 | 1.596 | 0.493-5.305 | 0.4312 | |
| Intermediate vs Mature | 1.527 | 0.733-3.202 | 0.2558 | 2.684 | 1.021-7.793 | 0.0452 | 1.895 | 0.696-5.686 | 0.2143 | ||||
| α-SMA | Positive vs Negative | 1.423 | 0.437-8.736 | 0.6078 | 1.580 | 0.333-28.264 | 0.6303 | ||||||
| CD10 | Positive vs Negative | 0.657 | 0.267-1.391 | 0.2883 | 0.704 | 0.295-1.941 | 0.4698 | ||||||
| Podoplamin | Positive vs Negative | 0.752 | 0.399-1.496 | 0.4020 | 0.564 | 0.251-1.343 | 0.1872 | ||||||
| FSP1 | Positive vs Negative | 0.355 | 0.108-2.185 | 0.2181 | 0.296 | 0.061-5.139 | 0.3172 | ||||||
| FAP | Positive vs Negative | 0.819 | 0.425-1.520 | 0.5323 | 4.045 | 0.853-72.358 | 0.0866 | ||||||
| Tenascin-C | Positive vs Negative | 5.025 | 2.164-14.621 | 0.0001 | 3.973 | 1.656-11.795 | 0.0012 | 4.527 | 1.559-19.172 | 0.0036 | 3.188 | 1.038-13.882 | 0.0421 |
| PDGFR-β | Positive vs Negative | 1.290 | 0.685-2.566 | 0.4395 | 1.416 | 0.611-3.661 | 0.4288 | ||||||
| ZEB1 | Positive vs Negative | 0.683 | 0.248-2.825 | 0.5463 | 0.562 | 0.166-3.510 | 0.4713 | ||||||
| TWIST1 | Positive vs Negative | 1.067 | 0.581-1.971 | 0.8331 | 1.237 | 0.553-2.818 | 0.6028 | ||||||
| Ki-67 | Positive vs Negative | 1.667 | 0.885-3.316 | 0.1157 | 2.952 | 1.187-8.906 | 0.0185 | 2.114 | 0.834-6.465 | 0.1191 | |||
| p53 | Positive vs Negative | 1.362 | 0.730-2.663 | 0.3378 | 1.255 | 0.552-3.096 | 0.5955 | ||||||
| MMP7 | Positive vs Negative | 0.651 | 0.354-1.199 | 0.1671 | 0.823 | 0.368-1.875 | 0.6358 | ||||||
| β-catenin | Positive vs Negative | 2.119 | 1.143-4.084 | 0.0169 | 1.502 | 0.796-2.940 | 0.2121 | 1.865 | 0.830-4.440 | 0.1323 | |||
| E-cadherin* | |||||||||||||
| HIF1-α* | |||||||||||||
WDA, Well-differentiated adenocarcinoma; MUC, mucinous carcinoma; HR, Hazard ratio; 95%CI, 95%confidence interval. *Could not analyze, why all cases were positive expression of the marker.