Figure 3.

miR-208b is secreted by colon cancer cells in the pattern of exosomes

(A) Transmission electron microscopy showed exosomes purified from the supernatants of NCM460, SW480, and SW480-OXA cells. (B) Nanoparticle tracking analysis was used to determine the absolute exosome size distribution and concentration of exosomes (particles/mL). (C) Western blot analysis of the expression of exosomal markers (CD63, TSG101, and Alix) in cell lysates and exosomal protein purified from NCM460, SW480, and SW480-OXA cell supernatants. (D) Relative levels of miR-208b in NCM460, SW480, and SW480-OXA cells measured by qRT-PCR (n = 3). (E) qRT-PCR analysis of miR-208b levels in exosomes purified from NCM460, SW480, and SW480-OXA cell supernatants (n = 3). ∗∗p < 0.01.