Figure 6.

PDCD4 is closely associated with Treg expansion

(A) Western blot analysis of PDCD4 expression in cells transfected with PDCD4 overexpression (OE.PDCD4), siRNA of PDCD4 (si.PDCD4), and si.PDCD4+miR-208b inhibitors (si.PDCD4 inhibitors.208b) (n = 3). (B) Quantitative analysis of (A) (n = 3). (C) Relative levels of PDCD4 mRNA in cells transfected with OE.PDCD4, si.PDCD4, and si.PDCD4 inhibitors.208b (n = 3). (D) Flow cytometry analysis percentage of CD4⁺CD25^highFoxp3⁺ Tregs in OE.PDCD4, si.PDCD4, and si.PDCD4⁺ inhibitors.208b groups (n = 3). ∗∗p < 0.01(E) Histogram analysis of (D) (n=3).