Figure 4.

CDNF reduces internalization of α-synuclein preformed fibrils (PFFs) in a dose-dependent manner

¹²⁵I-labeled α-synuclein PFFs were added to mouse hippocampal cultures (E16, DIV 7) together with CDNF at different concentrations (ng/mL) or persephin (ng/mL) as a negative control. The PFFs were allowed to be internalized into neurons for 60 min at 37°C, after which the cultures were washed twice with PBS and finally with acid wash that removes the unspecifically bound extracellular PFFs. After this, the cells were lysed with 1 N NaOH, and the radioactive signal from the internalized PFFs was counted using gamma counter. Kruskal-Wallis test = 18.79, number of groups = 7, p = 0.0045. Error bars represent SEM.